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タイトル: Alpha-glucosidase-like activity detected in a siboglinid polychaete, Oligobrachia mashikoi
著者: Koizumi, Takashi
Sasayama, Yuichi link image
小泉, 隆
笹山, 雄一
発行日: 2008年 4月
出版社(者): 日本動物学会
雑誌名: Zoological Science
ISSN: 0289-0003
巻: 25
号: 4
開始ページ: 364
終了ページ: 371
抄録: Siboglinid worms live on carbohydrates produced by symbiotic bacteria. In this study, α-glucosidase-like activity was detected in the surface of the body and in the trophosome of Oligobrachia mashikoi. The enzyme exhibiting this activity was partially purified by consecutively applying the crude enzyme extract to Con-A-Sepharose and Sephadex-200 HR columns. The enzyme sample thus obtained gave a single activity peak at a position corresponding to 550 kDa in the Sephadex-200 HR gel filtration column. The enzyme was active in the range of pH 6.0-8.0, with a maximum activity at around pH 6.5. It specifically hydrolyzed maltose, and was inhibited by voglibose and miglitol. Moreover, a glucose transporter 2-like protein was detected by immunohistochemical and Western-blotting analyses using anti-rat GLUT2 polyclonal antibody. These results raise the question how this unique species lives. © 2008 Zoological Society of Japan.
DOI: 10.2108/zsj.25.364
URI: http://hdl.handle.net/2297/10950
資料種別: Journal Article
版表示: publisher

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/10950



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