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タイトル: Double-stranded RNA-activated protein kinase (PKR) fused to green fluorescent protein induces apoptosis of human embryonic kidney cells: Possible role in the Fas signaling pathway
著者: Takizawa, Takenori
Tatematsu, Chizuru
Nakanishi, Yoshinobu link image link image
中西, 義信
発行日: 1999年
出版社(者): 日本生化学会 = Japanese Biochemical Society
雑誌名: Journal of Biochemistry
ISSN: 0021-924X
巻: 125
号: 2
開始ページ: 391
終了ページ: 398
キーワード: Apoptosis
EGFP
Fas
PKR
Protein kinase
抄録: PKR is an interferon-inducible, double-stranded (ds) RNA-activated serine/threonine protein kinase, and has been shown to play roles in viral pathogenesis, cell growth and apoptosis. We expressed PKR as a fusion protein with enhanced jellyfish green fluorescence protein (EGFP) in human embryonic kidney 293 cells to visualize the effect of PKR transfection. The EGFP-fusion construct with wild-type PKR showed both auto- and substrate-phosphorylation activities independent of dsRNA, indicating EGFP-PKR is constitutively active. The EGFP-construct with a mutant PKR with the first RNA binding domain deleted still possessed kinase activities. On the other hand, the EGFP-fusion with a catalytically inactive mutant of PKR with the substitution of K at 296 with R, which has been shown to have tumorigenic properties, did not possess kinase activities. Transfection of the constitutive active forms of EGFP-PKR constructs induced apoptosis in 293 cells without dsRNA, whereas the EGFP-fusion with the catalytically inactive mutant did not cause apoptosis but rather protected cells from Fas-induced cell death. In addition, Fas-stimulation increased endogenous PKR activities. These results constitute evidence that PKR is sufficient to induce apoptosis, and plays a role in Fas-mediated apoptosis.
URI: http://hdl.handle.net/2297/14558
関連URI: http://jb.oxfordjournals.org/cgi/content/abstract/125/2/391
資料種別: Journal Article
権利関係: Copyright © 1999 Japanese Biochemical Society
版表示: publisher
出現コレクション:2.査読済論文(薬)

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