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タイトル: Double-stranded RNA-activated protein kinase (PKR) fused to green fluorescent protein induces apoptosis of human embryonic kidney cells: Possible role in the Fas signaling pathway
著者: Takizawa, Takenori
Tatematsu, Chizuru
Nakanishi, Yoshinobu link image link image
中西, 義信
発行日: 1999年
出版社(者): 日本生化学会 = Japanese Biochemical Society
雑誌名: Journal of Biochemistry
ISSN: 0021-924X
巻: 125
号: 2
開始ページ: 391
終了ページ: 398
キーワード: Apoptosis
Protein kinase
抄録: PKR is an interferon-inducible, double-stranded (ds) RNA-activated serine/threonine protein kinase, and has been shown to play roles in viral pathogenesis, cell growth and apoptosis. We expressed PKR as a fusion protein with enhanced jellyfish green fluorescence protein (EGFP) in human embryonic kidney 293 cells to visualize the effect of PKR transfection. The EGFP-fusion construct with wild-type PKR showed both auto- and substrate-phosphorylation activities independent of dsRNA, indicating EGFP-PKR is constitutively active. The EGFP-construct with a mutant PKR with the first RNA binding domain deleted still possessed kinase activities. On the other hand, the EGFP-fusion with a catalytically inactive mutant of PKR with the substitution of K at 296 with R, which has been shown to have tumorigenic properties, did not possess kinase activities. Transfection of the constitutive active forms of EGFP-PKR constructs induced apoptosis in 293 cells without dsRNA, whereas the EGFP-fusion with the catalytically inactive mutant did not cause apoptosis but rather protected cells from Fas-induced cell death. In addition, Fas-stimulation increased endogenous PKR activities. These results constitute evidence that PKR is sufficient to induce apoptosis, and plays a role in Fas-mediated apoptosis.
URI: http://hdl.handle.net/2297/14558
関連URI: http://jb.oxfordjournals.org/cgi/content/abstract/125/2/391
資料種別: Journal Article
権利関係: Copyright © 1999 Japanese Biochemical Society
版表示: publisher

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/14558



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