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タイトル: Type III Cu mutants of Myrothecium verrucaria bilirubin oxidase
著者: Shimizu, Atsushi
Samejima, Tatsuya
Hirota, Shun
Yamaguchi, Shotaro
Sakurai, Nobuhiko
Sakurai, Takeshi link image link image
櫻井, 武
発行日: 2003年 6月 1日
出版社(者): 日本生化学会 = Japanese Biochemical Society
雑誌名: Journal of Biochemistry
ISSN: 0021-924X
巻: 133
号: 6
開始ページ: 767
終了ページ: 772
キーワード: Bilirubin oxidase
Multicopper oxidase
Trinuclear Cu center
抄録: Type III Cu ligand, His456 and His458, of Myrothecium verrucaria (MT-1) bilirubin oxidases (BO) [EC] were doubly mutated as to Lys, Asp, and Val. In spite of perturbation of the type III Cu centers, these mutants were pale blue or colourless when isolated. However, they became intense blue on reaction with reducing agents such as dithionite, ascorbate, hexacyanoferrate(II), and octacyanotangstate(IV) under air, or with an oxidizing agent such as hexacyanoferrate(III), indicating that they are in mixed forms when expressed in Aspergillus oryzae. His456.458Lys and His456.458Asp mutated as to potential coordinating groups showed weak BO and ferroxidase activities, while His 456.458Val mutated as to non-coordinating groups showed no enzyme activity at all.
DOI: 10.1093/jb/mvg098
URI: http://hdl.handle.net/2297/16743
関連URI: http://jb.oxfordjournals.org/cgi/content/abstract/133/6/767
資料種別: Journal Article
権利関係: © 2003 Japanese Biochemical Society
版表示: publisher

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/16743



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