DSpace width= university logo mark
Japanese | English 

KURA > C. 医薬保健学域・研究域/医・薬学部/医学系研究科 > c10. 学術雑誌掲載論文(医・保健) > 1. 査読済論文(医学・保健) >


ファイル 記述 サイズフォーマット
ME-PR-KAMBAAYASHI-Y-46.pdf363.1 kBAdobe PDF
タイトル: Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte
著者: Kambayashi, Yasuhiro link image link image
Thanh Binh, Nguyen
Asakura, Hiroki W.
Hibino, Yuri link image link image
Hitomi, Yoshiaki link image
Nakamura, Hiroyuki link image link image
Ogino, Keiki link image
神林, 康弘
日比野, 由利
人見, 嘉哲
中村, 裕之
発行日: 2009年 1月
出版社(者): 日本酸化ストレス学会 = SFRR Japan
雑誌名: Journal of Clinical Biochemistry and Nutrition
ISSN: 0912-0009
巻: 44
号: 1
開始ページ: 46
終了ページ: 51
キーワード: ABTS
Human plasma
Lag time
Total antioxidant capacity
抄録: In the present study, we tried to establish an efficient assay for total antioxidant capacity (TAC) in human plasma using a 96-well microplate. TAC was assessed using lag time by antioxidants against the myoglobin-induced oxidation of 2,2′-azino-di(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) with hydrogen peroxide, and expressed as Trolox equivalent. The linearity of the calibration curve with Trolox was maintained with the Trolox concentration range from 2.5 μM to 25 μM (R2 = 0.997). The assay was applied to the measurement of TAC in healthy human plasma. Coefficient of variation in intraday assay was 2.4%. Difference was not observed in interday assay. Plasma TAC of men ((569 ± 41) μM Trolox equivalent; n = 6) was higher than that of women ((430 ± 28) μM Trolox equivalent; n = 4). After the vegetable juice was drunk for 1 week, the increase in plasma TAC was observed in almost all the volunteers. In summary, we developed the efficient assay for plasma TAC using a 96-well microplate.
DOI: 10.3164/jcbn.08-162
URI: http://hdl.handle.net/2297/17052
関連URI: http://www.jstage.jst.go.jp/article/jcbn/44/1/44_46/_article
資料種別: Journal Article
権利関係: Copyright (c) 2008 by The Editorial Secretariat of JCBN
版表示: publisher
出現コレクション:1. 査読済論文(医学・保健)

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/17052



Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - ご意見をお寄せください