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タイトル: Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte
著者: Kambayashi, Yasuhiro link image link image
Ogino, Keiki link image
Takemoto, Kei
Imagama, Takashi
Takigawa, Tomoko
Kimura, Shingo
Hibino, Yuri link image link image
Hitomi, Yoshiaki link image
Nakamura, Hiroyuki link image link image
神林, 康弘
日比野, 由利
人見, 嘉哲
中村, 裕之
発行日: 2009年 1月
出版社(者): 日本酸化ストレス学会 = SFRR Japan
雑誌名: Journal of Clinical Biochemistry and Nutrition
ISSN: 0912-0009
巻: 44
号: 1
開始ページ: 95
終了ページ: 103
キーワード: (Di)bromotyrosine
Allergic disease
Eosinophil activation marker
Oxidative stress
Polyclonal antibody
抄録: (Di)bromotyrosine is formed by the specific reaction of eosinophil peroxidase and can be used as an eosinophil activation marker. In the present study, an antibody for (di)bromotyrosine in proteins was prepared to investigate the pathogenesis of eosinophil-related diseases such as allergic responses. A rabbit polyclonal antibody was raised against brominated keyhole limpet hemocyanin. The specificity of the antiserum was investigated with an enzyme-linked immunosorbent assay (ELISA). The antiserum recognized brominated bovine serum albumin (BSA) and dibromotyrosine-conjugated BSA. The antiserum also reacted with chlorinated BSA and di-iodotyrosine-conjugated BSA. Moreover, the specificity of the antiserum was investigated using competitive ELISA. Dibromotyrosine and di-iodotyrosine inhibited the recognition of brominated BSA by the antiserum. However, the recognition of brominated BSA by the antiserum was not inhibited by bromotyrosine, chlorotyrosine, iodotyrosine, nitrotyrosine, aminotyrosine, phosphotyrosine, or tyrosine. These results suggested that the epitope of the antiserum is dihalogenated tyrosine. Immunohistochemically, the antiserum stained brominated rat eosinophils but not chlorinated or nitrated eosinophils. In conclusion, an antiserum for dihalogenated protein was prepared. It is expected that the antiserum will be useful for the analysis of the pathogenesis of allergic diseases such as asthma and atopic dermatitis.
DOI: 10.3164/jcbn.08-196
URI: http://hdl.handle.net/2297/17053
関連URI: http://www.jstage.jst.go.jp/article/jcbn/44/1/44_95/_article
資料種別: Journal Article
権利関係: Copyright (c) 2008 by The Editorial Secretariat of JCBN
版表示: publisher
出現コレクション:1. 査読済論文(医学・保健)

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