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タイトル: Nesfatin-1 evokes Ca2+ signaling in isolated vagal afferent neurons via Ca2+ influx through N-type channels
著者: Iwasaki, Yusaku
Nakabayashi, Hajime link image
Kakei, Masafumi
Shimizu, Hiroyuki
Mori, Masatomo
Yada, Toshihiko
中林, 肇
発行日: 2009年12月18日
出版社(者): Elsevier
雑誌名: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
巻: 390
号: 3
開始ページ: 958
終了ページ: 962
キーワード: Cholecystokinin
Cytosolic Ca2+
N-type Ca2+ channel
Nodose ganglion
抄録: Nesfatin-1, processed from nucleobindin 2, is an anorexigenic peptide expressed in the brain and several peripheral tissues including the stomach and pancreas. Peripheral, as well as intracerebroventricular, administration of nesfatin-1 suppresses feeding behavior, though underlying mechanisms are unknown. In this study, we examined effects of nesfatin-1 on cytosolic Ca2+ concentration ([Ca2+]i) in the neurons isolated from the vagal afferent nodose ganglion of mice. Nesfatin-1 at 10-10-10-8 M increased [Ca2+]i in the isolated neurons in a concentration-dependent manner, and at 10-8 M it increased [Ca2+]i in 33 out of 263 (12.5%) neurons. These responses were inhibited under Ca2+-free conditions and by N-type Ca2+ channel blocker, ω-conotoxin GVIA. All the nesfatin-1-responsive neurons also exhibited [Ca2+]i responses to capsaicin and cholecystokinin-8. These results provide direct evidence that nesfatin-1 activates vagal afferent neurons by stimulating Ca2+ influx through N-type channels, demonstrating the machinery through which peripheral nesfatin-1 can convey signals to the brain. © 2009 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.bbrc.2009.10.085
URI: http://hdl.handle.net/2297/20509
関連URI: http://www.elsevier.com/locate/issn/0006291X
資料種別: Journal Article
版表示: author
出現コレクション:1. 査読済論文(医学・保健)

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/20509



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