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タイトル: In vitro differentiation of lineage-negative bone marrow cells into microglia-like cells
著者: Noto, Daisuke
Takahashi, Kazuya link image
Miyake, Sachiko
Yamada, Masahito link image link image
高橋, 和也
山田, 正仁
発行日: 2010年 4月
出版社(者): Federation of European Neuroscience Societies / Blackwell Publishing
雑誌名: European Journal of Neuroscience
ISSN: 0953-816X
巻: 31
号: 7
開始ページ: 1155
終了ページ: 1163
キーワード: Astrocyte
GFP mice
Primary mixed glial culture
TREM2
抄録: Microglia are believed to be the only resident immune cells in the CNS, originating from hematopoietic-derived myeloid cells and invading the CNS during development. However, the detailed mechanisms of differentiation and transformation of microglial cells are not fully understood. Here, we demonstrate that murine microglial cells show two morphological forms in vitro, namely, small round cells expressing CD11b, Iba1, triggering receptor expressing on myeloid cells-2 (TREM2), and weakly expressing major histocompatibility complex class II and large flat cells expressing only CD11b and Iba1. Moreover, lineage-negative bone marrow (LN) cells cultured with primary mixed glial culture cells could differentiate into only the small round microglia-like cells, despite the absence of CCR2 and Gr-1 expression. Addition of macrophage colony stimulating factor (M-CSF) to LN cell culture allowed the proliferation and expression of TREM2 in LN cells, and the addition of neutralizing anti-M-CSF antibodies suppressed the proliferation of LN cells despite the expression of TREM2. When LN cells were cultured with M-CSF, the number of small round cells in the culture was considerably low, indicating that the small round morphology of the immature cells is not maintained in the presence of only M-CSF. On the other hand, when LN cells were grown in the presence of astrocytes, the small round cells were maintained at a concentration of approximately 30% of the total population. Therefore, cell-cell contact with glial cells, especially astrocytes, may be necessary to maintain the small round shape of the immature cells expressing TREM2. © Federation of European Neuroscience Societies and Blackwell Publishing Ltd.
DOI: 10.1111/j.1460-9568.2010.07152.x
URI: http://hdl.handle.net/2297/24038
資料種別: Journal Article
版表示: author
出現コレクション:1. 査読済論文(医学・保健)

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