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タイトル: Increased expression of deoxyribonucleic acid methyltransferase gene in human astrocytic tumors
著者: Okada, Yoshie
Okada, Takashi
Nomura, Masayuki
Hayashi, Yutaka link image
Yamashima, Tetsumori link image
Yamashita, Junkoh
林, 裕
山嶋, 哲盛
山下, 純宏
発行日: 2000年
出版社(者): 日本脳神経外科学会
雑誌名: Neurologia Medico-Chirurgica
ISSN: 0470-8105
巻: 40
号: 11
開始ページ: 564
終了ページ: 571
キーワード: Deoxyribonucleic acid methyltransferase
In situ hybridization
Semiquantitative reverse transcriptase-polymerase chain reaction
抄録: The relationship between the grade of astrocytic tumor and the expression of deoxyribonucleic acid methyltransferase (DNA-MTase) gene was examined. The levels of DNA-MTase messenger ribonucleic acid (mRNA) were measured by semiquantitative reverse transcriptase-polymerase chain reaction in surgical specimens from 12 astrocytic tumors (4 astrocytomas, 6 anaplastic astrocytomas, and 2 glioblastomas) and two normal brain tissues, and in four glioma cell lines. Compared to normal brain tissues, the levels of DNA-MTase mRNA were increased by 16- to 55-fold in low grade astrocytomas, and significantly increased by 200- to 4500-fold in high grade astrocytomas (anaplastic astrocytomas and glioblastomas) and more than 4500-fold in glioma cell lines. In situ hybridization with paraffin-embedded surgical specimens of human astrocytic tumors showed DNA-MTase mRNA was abundantly expressed in high grade astrocytomas. The detection of increased DNA-MTase expression in astrocytic tumor indicates involvement in the tumorigenesis and suggests that blocking of this change with specific inhibitors may offer new therapeutic strategies for malignant astrocytic tumors.
DOI: 10.2176/nmc.40.564
URI: http://hdl.handle.net/2297/25178
関連URI: http://jns.umin.ac.jp/
資料種別: Journal Article
権利関係: Copyright© The Japan Neurosurgical Society
版表示: publisher
出現コレクション:1. 査読済論文(医学・保健)

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/25178



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