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タイトル: High-speed atomic force microscopy reveals rotary catalysis of rotor-less F1-ATPase
著者: Uchihashi, Takayuki link image
Iino, Ryota
Ando, Toshio link image link image
Noji, Hiroyuki
内橋, 貴之
飯野, 亮太
安藤, 敏夫
野地, 博行
発行日: 2011年 8月 5日
出版社(者): Science AAAS
雑誌名: Science
ISSN: 0036-8075
巻: 333
号: 6043
開始ページ: 755
終了ページ: 758
抄録: F1 is an adenosine triphosphate (ATP)–driven motor in which three torque-generating β subunits in the α3β3 stator ring sequentially undergo conformational changes upon ATP hydrolysis to rotate the central shaft γ unidirectionally. Although extensive experimental and theoretical work has been done, the structural basis of cooperative torque generation to realize the unidirectional rotation remains elusive. We used high-speed atomic force microscopy to show that the rotorless F1 still “rotates”; in the isolated α3β3 stator ring, the three β subunits cyclically propagate conformational states in the counterclockwise direction, similar to the rotary shaft rotation in F1. The structural basis of unidirectionality is programmed in the stator ring. These findings have implications for cooperative interplay between subunits in other hexameric ATPases.
DOI: 10.00610.1126/science.1205510
URI: http://hdl.handle.net/2297/28580
関連URI: http://www.sciencemag.org/
資料種別: Journal Article
版表示: author

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/28580



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