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タイトル: ラット腹水肝癌AH66細胞におけるビンブラスチンの膜透過機序の検討
その他のタイトル: Studies on Mechanisms of Vinblastine Membrane Transport in Rat Ascites Hepatoma AH66 Cells
著者: 涌沢, 伸哉
柳岡, 利一
宮本, 謙一
越浦, 良三
Wakusawa, Shinya
Yanaoka, Toshikazu
Miyamoto, Kenichi
Koshiura, Ryozo
発行日: 1984年12月25日
出版社(者): 日本薬学会
引用: 藥學雜誌 104(12), pp.1301-1305
雑誌名: 藥學雜誌
ISSN: 0031-6903
巻: 104
号: 12
開始ページ: 1301
終了ページ: 1305
キーワード: vinblastine
Ca^<2+> antagonist
metabolic inhibitor
membrane transport
AH66 cell
抄録: The influence of Ca^<2+> antagonists and metabolic inhibitors on the membrane transport of vinblastine (VBL) in rat ascites hepatoma AH66 cells was investigated. Such Ca^<2+> antagonists as ruthenium red (RR) and lanthanum chloride (La) decreased the uptake of VBL but did not influence the intracellular retention of VBL. In contrast, verapamil markedly increased the uptake and decreased the efflux of VBL. Since AH66 cells are not excitable cells, it was considered that verapamil which is a Ca^<2+> channel antagonist showed a different action from other Ca^<2+> antagonists. Iodoacetic acid increased the uptake of VBL and suppressed the extrusion of VBL but dinitrophenol and ouabain did not influence the intracellular content of VBL in the glucose rich medium. On the other hand, RR and La specifically inhibited the activity of (Ca^<2+>-Mg^<2+>) ATPase. Ouabain also specifically inhibited the activity of (Na^+-K^+) ATPase. Iodoacetic acid suppressed the activities of ATPase except (Na^+-K^+) ATPase. Therefore, in the influx of VBL, the presence of Ca^<2+> on the plasma membrane or in the cell seemed to be necessary. Moreover, it was suggested that VBL was not extruded by neither (Na^+-K^+) ATPase nor (Ca^<2+>-Mg^<2+>) ATPase in the plasma membrane and a different active transport system might be concerned with the efflux of VBL.
URI: http://hdl.handle.net/2297/7050
資料種別: Journal Article
版表示: publisher
出現コレクション:1. 査読済論文

このアイテムを引用あるいはリンクする場合は次の識別子を使用してください。 http://hdl.handle.net/2297/7050



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